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GSH reacts with dithionitrobenzoic acid (DTNB) and produce GS-TNB and TNB anion that can be detected either fluorometric or colorimetric, the rate of TNB production is proportional to the amount of GSH

Although the drop in hepatic or renal GSH is the most toxicologically relevant interaction (see also below), plasma GSH, free cysteine (Burgunder et al., 1989), and antioxidant capacity (Nuttall et al., 2003) were significantly reduced after a single 2 g PAC administration or 14 days of therapeutic doses of PAC in human volunteers, respectively

Your liver produces it naturally, and many different systems in your body use it daily

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